Bioinformatic and expression analysis of the Brassica napus L. cyclophilins.
Fiche publication
Date publication
mai 2017
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HEINLEIN Manfred
Tous les auteurs :
Hanhart P, Thieß M, Amari K, Bajdzienko K, Giavalisco P, Heinlein M, Kehr J
Lien Pubmed
Résumé
Cyclophilins (CYPs) are a group of ubiquitous proteins characterized by their ability to bind to the immunosuppressive drug cyclosporin A. The CYP family occurs in a wide range of organisms and contains a conserved peptidyl-prolyl cis/trans isomerase domain. In addition to fulfilling a basic role in protein folding, CYPs may also play diverse important roles, e.g. in protein degradation, mRNA processing, development, and stress responses. We performed a genome-wide database survey and identified a total of 94 CYP genes encoding 91 distinct proteins. Sequence alignment analysis of the putative BnCYP cyclophilin-like domains revealed highly conserved motifs. By using RNA-Seq, we could verify the presence of 77 BnCYP genes under control conditions. To identify phloem-specific BnCYP proteins in a complementary approach, we used LC-MS/MS to determine protein abundances in leaf and phloem extracts. We detected 26 BnCYPs in total with 12 being unique to phloem sap. Our analysis provides the basis for future studies concentrating on the functional characterization of individual members of this gene family in a plant of dual importance: as a crop and a model system for polyploidization and long-distance signalling.
Mots clés
Amino Acid Sequence, Arabidopsis Proteins, chemistry, Brassica napus, genetics, Computational Biology, methods, Conserved Sequence, Cyclophilins, chemistry, Gene Expression Regulation, Plant, Genes, Plant, Genome, Plant, Phloem, genetics, Phylogeny, Plant Leaves, genetics, Plant Proteins, chemistry, Protein Domains, RNA, Messenger, genetics, Structural Homology, Protein, Subcellular Fractions, metabolism
Référence
Sci Rep. 2017 05 4;7(1):1514