Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread.
Fiche publication
Date publication
mai 2014
Journal
Virology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves, Dr HEINLEIN Manfred
Tous les auteurs :
Niehl A, Pasquier A, Ferriol I, Mély Y, Heinlein M
Lien Pubmed
Résumé
Tobacco mosaic virus (TMV) is a longstanding model for studying virus movement and macromolecular transport through plasmodesmata (PD). Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral replication complexes (VRCs) along the ER-actin network towards PD. To investigate whether this movement mechanism might be conserved between tobamoviruses, we compared the functions of Oilseed rape mosaic virus (ORMV) MP with those of MP(TMV). We show that MP(ORMV) supports TMV movement more efficiently than MP(TMV). Moreover, MP(ORMV) localizes to C-MERs like MP(TMV) but accumulates to lower levels and does not localize to larger inclusions/VRCs or along MTs, patterns regularly seen for MP(TMV). Our findings extend the role of C-MERs in viral cell-to-cell transport to a virus commonly used for functional genomics in Arabidopsis. Moreover, accumulation of tobamoviral MP in inclusions or along MTs is not required for virus movement.
Mots clés
Arabidopsis, virology, Endoplasmic Reticulum, metabolism, Inclusion Bodies, Viral, Microtubules, metabolism, Plant Viral Movement Proteins, genetics, Tobamovirus, genetics
Référence
Virology. 2014 May;456-457:43-54