In vitro impact of physiological shear stress on endothelial cells gene expression profile.
Fiche publication
Date publication
janvier 2007
Journal
Clinical hemorheology and microcirculation
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MENU Patrick, Pr BOURA Cédric
Tous les auteurs :
Gaucher C, Devaux C, Boura C, Lacolley P, Stoltz JF, Menu P
Lien Pubmed
Résumé
In the vascular system, the shear applied to the vascular wall activates mechano-sensors located on endothelial cells (ECs) leading to a modification in the gene expression profile. We applied laminar shear stress at 1 Pa on ECs for 6 h and measured by quantitative real time PCR the expression modulation of genes implied in inflammation (ICAM-1 and E-selectin), oxidative stress sensing (HO-1) and vascular tone modulation (eNOS). We showed that all these genes are shear stress inducible. ICAM-1 is more up-regulated than E-selectin suggesting different levels of implication in inflammatory responses and different modes of induction (SSRE, cytokine). Laminar shear stress induces an oxidative stress translated into HO-1 up-regulation, and a possible vasodilatation through the induction of eNOS. Our laminar shear stress system opens a novel and interesting frame in the evaluation of the impact on ECs and blood cells of new pharmacological substances injected in the bloodstream.
Mots clés
Cells, Cultured, Endothelial Cells, cytology, Endothelium, Vascular, cytology, Gene Expression Profiling, Humans, Inflammation, genetics, Oxidative Stress, genetics, Stress, Mechanical, Umbilical Veins, cytology, Up-Regulation, genetics, Vasoconstriction, genetics
Référence
Clin. Hemorheol. Microcirc.. 2007 ;37(1-2):99-107