Repertoire and neutralizing activity of antibodies against E2 peptide in patients with spontaneous resolution of hepatitis C.
Fiche publication
Date publication
juin 2019
Journal
The Journal of infectious diseases
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BAUMERT Thomas
Tous les auteurs :
Olbrich A, Wardemann H, Böhm S, Rother K, Colpitts CC, Wrensch F, Baumert TF, Berg T, Benckert J
Lien Pubmed
Résumé
Neutralizing antibodies can prevent hepatitis C virus (HCV) infection, one of the leading causes of cirrhosis and liver cancer. Here, we characterized the immunoglobulin repertoire of memory B cell antibodies against a linear epitope in the central front layer of HCV envelope (E2) (aa483-499) in patients that were infected in a single-source outbreak. A reverse transcriptase (RT)-PCR based immunoglobulin gene cloning and recombinant expression approach was applied to express monoclonal antibodies from HCV E2-peptide-binding IgG+ memory B cells. We identified highly mutated antibodies with neutralizing effect in vitro against different genotype isolates sharing similar gene features. Our data confirm the importance of VH1-69 usage for neutralizing activity. The data offers a promising basis for vaccine research and the use of anti-E2 antibodies as a mean of passive immunization.
Mots clés
HCV, HCV envelope (E2) protein, anti-D cohort, memory B cells, neutralizing antibodies
Référence
J. Infect. Dis.. 2019 Jun 4;: