Transcriptional regulation of the Glutamate/GABA/Glutamine cycle in adult glia controls motor activity and seizures in .
Fiche publication
Date publication
mai 2019
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GIANGRANDE Angela
Tous les auteurs :
Mazaud D, Kottler B, Gonçalves-Pimentel C, Proelss S, Tüchler N, Deneubourg C, Yuasa Y, Diebold C, Jungbluth H, Lai EC, Hirth F, Giangrande A, Fanto M
Lien Pubmed
Résumé
The fruitfly has been extensively used as a genetic model for the maintenance of nervous system's functions. Glial cells are of utmost importance in regulating the neuronal functions in the adult organism and in the progression of neurological pathologies. Through a microRNA-based screen in adult glia, we uncovered the essential role of a major glia developmental determinant, , in the adult fly. Here, we report that Repo expression is continuously required in adult glia to transcriptionally regulate the highly conserved function of neurotransmitter recycling in both males and females. Transient loss of Repo dramatically shortens fly lifespan, triggers motor deficits and increases the sensibility to seizures, partly due to the impairment of the Glutamate/GABA/Glutamine cycle. Our findings highlight the pivotal role of transcriptional regulation of genes involved in the Glutamate/GABA/Glutamine cycle in glia to control neurotransmitter levels in neurons and their behavioural output. The mechanism here identified in exemplifies how adult functions can be modulated at the transcriptional level and suggest an active synchronised regulation of genes involved in the same pathway. The process of neurotransmitter recycling is of essential importance in human epileptic and psychiatric disorders and our findings may thus have important consequences for the understanding of the role that transcriptional regulation of neurotransmitter recycling in astrocytes has in human disease.Glial cells are an essential support to neurons in adult life and have been involved in a number of neurological disorders. What controls the maintenance and modulation of glial functions in adult life is not fully characterized. Through a miR-overexpression screen in adult glia in , we identify an essential role in adult glia of which directs glial differentiation during embryonic development. Repo levels modulate, via transcriptional regulation, the ability of glial cells to support neurons in the Glutamate/GABA/Glutamine cycle. This leads to significant abnormalities in motor behaviour as assessed through a novel automated paradigm. Our work points to the importance of transcriptional regulation in adult glia for neurotransmitter recycling, a key process in several human neurological disorders.
Référence
J. Neurosci.. 2019 May 7;: