Matrix remodeling and MMP expression/activation is associated with hidradenitis suppurativa skin inflammation.
Fiche publication
Date publication
mars 2019
Journal
Experimental dermatology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr ANTONICELLI Franck
Tous les auteurs :
Sanchez J, Le Jan S, Muller C, François C, Renard Y, Durlach A, Bernard P, Reguiai Z, Antonicelli F
Lien Pubmed
Résumé
Hidradenitis suppurativa/acne inversa (HS) is a chronic, inflammatory, recurrent, debilitating skin disease of the hair follicle, associated with considerable tissue remodeling. Although abnormal cytokines expression was detected both in perilesional and in uninvolved skin, up to now there is no model allowing a better understanding of the implicit inflammatory mechanisms in HS. The aim of this study was to investigate the inflammatory response in HS skin by mean of an ex vivo model culture. To that purpose, 9 skin biopsy specimens from patients suffering from HS and controls were cultured up to 4 days. Microscopy imaging investigations showed variations of collagen I and III organization, and an increase in elastin fibers fragmentation in HS skin after 4 days of culture. The HS matrix structure remodeling was associated with high level of MMP-2 and MMP-9 in HS lesional skin. After 4 days of culture, the MMPs expression in HS perilesional skin reached the level observed in HS lesional skin. Concomitantly, an increase in IL-1β concentration was observed in all skin samples after 4 days of culture, although IL-1β concentrations remained significantly higher in HS lesional skin as compared with control skin. Meanwhile, neither IL-17 concentrations nor the inflammasome components NLRP3 and CASPase-1 varied. Thus, our HS skin model culture showed that MMP-induced matrix alteration could participate to HS inflammation by releasing biological active peptides and inflammatory factors from the extracellular matrix (ECM), and open new opportunities to investigate the regulation of the inflammatory mechanism associated with HS. This article is protected by copyright. All rights reserved.
Mots clés
cytokines, hidradenitis suppurativa, inflammation, skin architecture
Référence
Exp. Dermatol.. 2019 Mar 23;: