Two-dimensional mass spectrometry: new perspectives for tandem mass spectrometry.

Fiche publication


Date publication

mars 2019

Journal

European biophysics journal : EBJ

Auteurs

Membres identifiés du Cancéropôle Est :
Dr DELSUC Marc-André


Tous les auteurs :
van Agthoven MA, Lam YPY, O'Connor PB, Rolando C, Delsuc MA

Résumé

Fourier transform ion cyclotron resonance mass analysers (FT-ICR MS) can offer the highest resolutions and mass accuracies in mass spectrometry. Mass spectra acquired in an FT-ICR MS can yield accurate elemental compositions of all compounds in a complex sample. Fragmentation caused by ion-neutral, ion-electron, or ion-photon interactions leads to more detailed structural information on compounds. The most often used method to correlate compounds and their fragment ions is to isolate the precursor ions from the sample before fragmentation. Two-dimensional mass spectrometry (2D MS) offers a method to correlate precursor and fragment ions without requiring precursor isolation. 2D MS therefore enables easy access to the fragmentation patterns of all compounds from complex samples. In this article, the principles of FT-ICR MS are reviewed and the 2D MS experiment is explained. Data processing for 2D MS is detailed, and the interpretation of 2D mass spectra is described.

Mots clés

Fourier transform, Fourier transform ion cyclotron resonance mass spectrometry, Mass spectrometry, Tandem mass spectrometry, Two dimensional

Référence

Eur. Biophys. J.. 2019 Mar 13;: