Histone H3.3 regulates mitotic progression in mouse embryonic fibroblasts.
Fiche publication
Date publication
février 2017
Journal
Biochemistry and cell biology = Biochimie et biologie cellulaire
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HAMICHE Ali
Tous les auteurs :
Ors A, Papin C, Favier B, Roulland Y, Dalkara D, Ozturk M, Hamiche A, Dimitrov S, Padmanabhan K
Lien Pubmed
Résumé
H3.3 is a histone variant, which marks transcription start sites as well as telomeres and heterochromatic sites on the genome. H3.3 presence is thought to positively correlate with transcriptional status of its target genes. Using a conditional genetic strategy against H3.3B combined with short hairpin RNAs against H3.3A, we essentially depleted all H3.3 gene expression in mouse embryonic fibroblasts. Following nearly complete loss of H3.3 in cells, our transcriptomic analyses show very little impact on global gene expression as well as on histone variant H2A.Z localization. Instead, fibroblasts display slower cell growth and an increase in cell death coincident with large-scale chromosome misalignment in mitosis and large polylobed or micronuclei in interphase cells. Thus we conclude that H3.3 may additionally have an important under-explored role in chromosome segregation, nuclear structure and maintenance of genome integrity.
Mots clés
H3.3, RNA-seq, fibroblastes embryonnaires de souris, mitose, mitosis, mouse embryonic fibroblasts, transcription
Référence
Biochem. Cell Biol.. 2017 Feb;: