Harnessing Wharton's jelly stem cell differentiation into bone-like nodule on calcium phosphate substrate without osteoinductive factors.
Fiche publication
Date publication
février 2017
Journal
Acta biomaterialia
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr VOEGEL Jean-Claude, Dr FRANCIUS Grégory, Dr BOULAGNON-ROMBI Camille, Dr VELARD Frédéric, Pr SCHAAF Pierre, Dr KERDJOUDJ Halima
Tous les auteurs :
Mechiche Alami S, Rammal H, Boulagnon-Rombi C, Velard F, Lazar F, Drevet R, Laurent Maquin D, Gangloff SC, Hemmerlé J, Voegel JC, Francius G, Schaaf P, Boulmedais F, Kerdjoudj H
Lien Pubmed
Résumé
An important aim of bone regenerative medicine is to design biomaterials with controlled chemical and topographical features to guide stem cell fate towards osteoblasts without addition of specific osteogenic factors. Herein, we find that sprayed bioactive and biocompatible calcium phosphate substrates (CaP) with controlled topography induce, in a well-orchestrated manner, Wharton's jelly stem cells (WJ-SCs) differentiation into osteoblastic lineage without any osteogenic supplements. The resulting WJ-SCs commitment exhibits features of native bone, through the formation of three-dimensional bone-like nodule with osteocyte-like cells embedded into a mineralized type I collagen. To our knowledge, these results present the first observation of a whole differentiation process from stem cell to osteocytes-like on a synthetic material. This suggests a great potential of sprayed CaP and WJ-SCs in bone tissue engineering. These unique features may facilitate the transition from bench to bedside and the development of successful engineered bone.
Mots clés
Bone regeneration, Bone-like nodule, Calcium phosphate, Osteogenic factors free, Wharton’s jelly stem cells
Référence
Acta Biomater. 2017 Feb;49:575-589