Evaluation of mTHPC-loaded PLGA nanoparticles for in vitro photodynamic therapy on C6 glioma cell line.
Fiche publication
Date publication
janvier 2019
Journal
Photodiagnosis and photodynamic therapy
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ANDRY Marie-Christine, Dr TERRYN Christine
Tous les auteurs :
Bœuf-Muraille G, Rigaux G, Callewaert M, Zambrano N, Van Gulick L, Roullin VG, Terryn C, Andry MC, Chuburu F, Dukic S, Molinari M
Lien Pubmed
Résumé
Photodynamic therapy (PDT) is a very attractive strategy to complement or replace common cancer treatments such as radiotherapy, surgery, and chemotherapy. If some molecules have shown their efficiency as photosensitizers (PS), many issues have to be solved such as the inherent cytotoxicity of the PS or its hydrophobic properties causing limitation in their solubility and leading to side effects. In this study, the encapsulation of an approved PS, the meso-tetra hydroxyphenylchlorine (mTHPC, Foscan®) within biocompatible and biodegradable poly(D, L-lactide-co-glycolide) acid (PLGA) NPs prepared by the nanoprecipitation method was studied. The mTHPC-loaded NPs (mTHPC ⊂ PLGA NPs) were analyzed by UV-Visible spectroscopy to determine the efficiency of mTHPC encapsulation, and by dynamic light scattering (DLS) and atomic force microscopy (AFM) to determine mTHPC ⊂ PLGA NPs sizes, morphologies and surface charges. The longitudinal follow-up of mTHPC release from the NPs indicated that 50% of the encapsulated PS was retained within the NP matrix after a period of five days. Finally, the cytotoxicity and the phototoxicity of the mTHPC ⊂ PLGA NPs were determined in murine C6 glioma cell lines and compared to the ones of mTHPC alone. The studies showed a strong decrease of mTHPC cytotoxicity and an increase of mTHPC photo-cytotoxicity when mTHPC was encapsulated. In order to have a better insight of the underlying cellular mechanisms that governed cell death after mTHPC ⊂ PLGA NPs incubation and irradiation, annexin V staining tests were performed. The results indicated that apoptosis was the main cell death mechanism.
Mots clés
Apoptosis, Cell death mechanism, PLGA nanoparticles, Photodynamic therapy, mTHPC encapsulation
Référence
Photodiagnosis Photodyn Ther. 2019 Jan 29;: