Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis.
Fiche publication
Date publication
janvier 2019
Journal
RNA (New York, N.Y.)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHARPENTIER Bruno, Dr MASSENET Séverine
Tous les auteurs :
Paul A, Tiotiu D, Bragantini B, Marty H, Charpentier B, Massenet S, Labialle S
Lien Pubmed
Résumé
Deciphering the assembly of functional RNPs is a highly active field of RNA biology, especially due to the complexity of known assembly systems and their importance for cell function. C/D box snoRNP biogenesis is conserved in eukaryotes and is essential for correct ribosome maturation. To date, the Bcd1 protein has been shown to be strictly required for the specific accumulation of box C/D snoRNAs in yeast and human, but its role is unknown. In this context, the manuscript reports a functional analysis of the Bcd1 protein in budding yeast based on chromatin, RNA and protein immunoprecipition assays as well as genetic approaches. Using these methodologies is original and complementary to previous studies as current biogenesis models have been built by integrating information coming mainly from protein-protein interaction analyses. It allows to present in an unprecedented view the ordered loading of snoRNP-related proteins on immature and mature RNA species and to show that C/D box snoRNP biogenesis initiates co-transcriptionally. It evidences that Bcd1p is a master and early controller of this process that directs the loading of the core protein Nop58 on RNA. Therefore, we believe that this work fuels significantly our understanding of eukaryotic C/D box snoRNP biogenesis and bring general new insight on the essential mechanism of RNP assembly in cells.
Mots clés
BCD1/Bcd1p (ZNHIT6), C/D box snoRNA, NOP58/Nop58p, Saccharomyces cerevisiae, small noncoding RNA, small nucleolar ribonucleoprotein
Référence
RNA. 2019 Jan 30;: