Bioinspired Nanofeatured Substrates: Suitable Environment for Bone Regeneration.
Fiche publication
Date publication
mars 2017
Journal
ACS applied materials & interfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr FRANCIUS Grégory, Dr TERRYN Christine, Pr SCHAAF Pierre, Dr KERDJOUDJ Halima, Pr ALEM-MARCHAND Halima
Tous les auteurs :
Rammal H, Dubus M, Aubert L, Reffuveille F, Laurent-Maquin D, Terryn C, Schaaf P, Alem H, Francius G, Quilès F, Gangloff SC, Boulmedais F, Kerdjoudj H
Lien Pubmed
Résumé
Bone mimicking coatings provide a complex microenvironment in which material, through its inherent properties (such as nanostructure and composition), affects the commitment of stem cells into bone lineage and the production of bone tissue regulating factors required for bone healing and regeneration. Herein, a bioactive mineral/biopolymer composite made of calcium phosphate/chitosan and hyaluronic acid (CaP-CHI-HA) was elaborated using a versatile simultaneous spray coating of interacting species. The resulting CaP-CHI-HA coating was mainly constituted of bioactive, carbonated and crystalline hydroxyapatite with 277 ± 98 nm of roughness, 1 μm of thickness, and 2.3 ± 1 GPa of stiffness. After five days of culture, CaP-CHI-HA suggested a synergistic effect of intrinsic biophysical features and biopolymers on stem cell mechanobiology and nuclear organization, leading to the expression of an early osteoblast-like phenotype and the production of bone tissue regulating factors such as osteoprotegerin and vascular endothelial growth factor. More interestingly, amalgamation with biopolymers conferred to the mineral a bacterial antiadhesive property. These significant data shed light on the potential regenerative application of CaP-CHI-HA bioinspired coating in providing a suitable environment for stem cell bone regeneration and an ideal strategy to prevent implant-associated infections.
Mots clés
bioinspired coating, bone regeneration, human stem cells, mechanobiology, nanomedicine, stem cell differentiation
Référence
ACS Appl Mater Interfaces. 2017 Mar;: