Abrogated Cell Contact Guidance on Amino-Functionalized Microgrooves.
Fiche publication
Date publication
mars 2017
Journal
ACS applied materials & interfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ANSELME Karine
Tous les auteurs :
Mörke C, Rebl H, Finke B, Dubs M, Nestler P, Airoudj A, Roucoules V, Schnabelrauch M, Körtge A, Anselme K, Helm CA, Nebe JB
Lien Pubmed
Résumé
Topographical and chemical features of biomaterial surfaces affect the cell physiology at the interface and are promising tools for the improvement of implants. The dominance of the surface topography on cell behavior is often accentuated. Striated surfaces induce an alignment of cells and their intracellular adhesion-mediated components. Recently, it could be demonstrated that a chemical modification via plasma polymerized allylamine was not only able to boost osteoblast cell adhesion and spreading but also override the cell alignment on stochastically machined titanium. In order to discern what kind of chemical surface modifications let the cell forget the underlying surface structure, we used an approach on geometric microgrooves produced by deep reactive ion etching (DRIE). In this study, we systematically investigated the surface modification by (i) methyl-, carboxyl-, and amino functionalization created via plasma polymerization processes, (ii) coating with the extracellular matrix protein collagen-I or immobilization of the integrin adhesion peptide sequence Arg-Gly-Asp (RGD), and (iii) treatment with an atmospheric pressure plasma jet operating with argon/oxygen gas (Ar/O2). Interestingly, only the amino functionalization, which presented positive charges at the surface, was able to chemically disguise the microgrooves and therefore to interrupt the microtopography induced contact guidance of the osteoblastic cells MG-63. However, the RGD peptide coating revealed enhanced cell spreading as well, with fine, actin-containing protrusions. The Ar/O2-functionalization demonstrated the best topography handling, e.g. cells closely attached even to features such as the sidewalls of the groove steps. In the end, the amino functionalization is unique in abrogating the cell contact guidance.
Mots clés
RGD, actin cytoskeleton, contact guidance, correlative microscopy, geometric microgrooves, physical plasma, water contact angle, zeta potential
Référence
ACS Appl Mater Interfaces. 2017 Mar;9(12):10461-10471