Identification of autoreactive B cells with labeled nucleosomes.
Fiche publication
Date publication
avril 2017
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr WAGNER Alain, Dr DUMORTIER Hélène, Pr MARTIN Thierry
Tous les auteurs :
Gies V, Wagner A, Seifert C, Guffroy A, Fauny JD, Knapp AM, Pasquali JL, Martin T, Dumortier H, Korganow AS, Soulas-Sprauel P
Lien Pubmed
Résumé
The pathogenesis of autoimmune diseases has not been completely elucidated yet, and only a few specific treatments have been developed so far. In autoimmune diseases mediated by pathogenic autoantibodies, such as systemic lupus erythematosus, the specific detection and analysis of autoreactive B cells is crucial for a better understanding of the physiopathology. Biological characterization of these cells may help to define new therapeutic targets. Very few techniques allowing the precise detection of autoreactive B cells have been described so far. Herein we propose a new flow cytometry technique for specific detection of anti-nucleosome B cells, which secrete autoantibodies in systemic lupus erythematosus, using labeled nucleosomes. We produced different fluorochrome-labeled nucleosomes, characterized them, and finally tested them in flow cytometry. Nucleosomes labeled via the cysteines present in H3 histone specifically bind to autoreactive B cells in the anti-DNA transgenic B6.56R mice model. The present work validates the use of fluorochrome-labeled nucleosomes via cysteines to identify anti-nucleosome B cells and offers new opportunities for the description of autoreactive B cell phenotype.
Mots clés
Animals, Autoantibodies, immunology, Autoantigens, immunology, Autoimmunity, B-Lymphocytes, immunology, Biomarkers, Cell Line, Female, Flow Cytometry, Humans, Lupus Erythematosus, Systemic, immunology, Mice, Nucleosomes, immunology, Staining and Labeling
Référence
Sci Rep. 2017 Apr;7(1):602