Selective Luminescent Labeling of DNA and RNA Quadruplexes by π-Extended Ruthenium Light-Up Probes.
Fiche publication
Date publication
avril 2017
Journal
Chemistry (Weinheim an der Bergstrasse, Germany)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MONCHAUD David
Tous les auteurs :
Saadallah D, Bellakhal M, Amor S, Lefebvre JF, Chavarot-Kerlidou M, Baussanne I, Moucheron C, Demeunynck M, Monchaud D
Lien Pubmed
Résumé
A series of Ru complexes exhibiting π-extended, acridine-based ancillary chelating heterocycles display high affinity and selectivity for DNA and RNA quadruplexes. The most promising candidates (3, 4) possess remarkable light-up luminophore properties (up to 330-fold luminescence enhancement upon interaction with quadruplexes), enabling them to discriminate quadruplexes from genomic DNA owing to a photochemical mechanism involving DNA protection against non-radiative decay (DAND), thus deviating from the other complexes of this series of ligands that exhibit an excited-state intramolecular proton transfer (ESIPT) that quenches their luminescence. The in vitro and preliminary in cellulo results shown here confirm the interest of this new family of fluorophores as invaluable molecular tools to detect G-quadruplexes in cells.
Mots clés
DNA, chemistry, Fluorescent Dyes, chemistry, G-Quadruplexes, Ligands, Organometallic Compounds, chemistry, RNA, chemistry, Ruthenium, chemistry
Référence
Chemistry. 2017 Apr 11;23(21):4967-4972