Detecting RNA modifications in the epitranscriptome: predict and validate.
Fiche publication
Date publication
mai 2017
Journal
Nature reviews. Genetics
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri
Tous les auteurs :
Helm M, Motorin Y
Lien Pubmed
Résumé
RNA modifications are emerging players in the field of post-transcriptional regulation of gene expression, and are attracting a comparable degree of research interest to DNA and histone modifications in the field of epigenetics. We now know of more than 150 RNA modifications and the true potential of a few of these is currently emerging as the consequence of a leap in detection technology, principally associated with high-throughput sequencing. This Review outlines the major developments in this field through a structured discussion of detection principles, lays out advantages and drawbacks of new high-throughput methods and presents conventional biophysical identification of modifications as meaningful ways for validation.
Mots clés
Animals, Epigenesis, Genetic, genetics, Epigenomics, methods, Gene Expression Regulation, Humans, RNA, chemistry, RNA Processing, Post-Transcriptional, Transcriptome, genetics
Référence
Nat. Rev. Genet.. 2017 05;18(5):275-291