CpG island methylation correlates with the use of alternative promoter for USP44 gene expression in human pluripotent stem cells and testis.
Fiche publication
Date publication
mai 2017
Journal
Stem cells and development
Auteurs
Membres identifiés du Cancéropôle Est :
Pr VIVILLE Stéphane
Tous les auteurs :
Tropel P, Jung L, André C, Ndandougou A, Viville S
Lien Pubmed
Résumé
Deubiquitinating enzymes may play a major regulatory role in pluripotent stem cells (PSCs) but few studies have investigated this topic. Within this family of enzymes, we found that the ubiquitin specific peptidase, USP44, is highly expressed in embryonic stem cells, induced PSCs and testes as compared to differentiated progenies and somatic organs. Analysis by qPCR and 5'RACE showed that alternate promoters are responsible for expression in PSCs and organs. We noticed 7 regions of transcription initiation, some of them with cell- or tissue-specific activity. Close analysis showed that one of the promoters involved in stem cell and testis-specific activity is differentially regulated in those tissues. At the epigenetic level, USP44 transcription was correlated with DNA methylation of a CpG island close to the main promoter region. These data imply a complex picture where regulating factors like OCT4 may interact with other epigenetic mechanisms to regulate USP44 expression in PSCs and testes.
Mots clés
DNA methylation, USP44, pluripotent stem cells, promoter, regulation, transcription initiation start
Référence
Stem Cells Dev.. 2017 May;: