Artocarpin-enriched (Artocarpus altilis) Heartwood Extract Protects Against UVB-induced Mechanical Damage in Dermal Fibroblasts.
Fiche publication
Date publication
mai 2017
Journal
Photochemistry and photobiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HUMBERT Philippe
Tous les auteurs :
Tiraravesit N, Humbert P, Robin S, Tissot M, Viennet C, Viyoch J
Lien Pubmed
Résumé
Ultraviolet B radiation has a negative effect on the mechanical properties of the dermis and on the activities of resident fibroblasts, including the disorganization and fragmentation of the dermal extracellular matrix (ECM). It induces alterations in cytoskeleton structure, motility and the contractile forces of the fibroblasts. The present study aimed to evaluate the protective effect of Artocarpus altilis heartwood extract on the mechanical properties of ultraviolet B-irradiated fibroblasts. Human skin fibroblasts were pretreated with 50 μg/mL artocarpin-enriched extract for 48 h and later irradiated with ultraviolet B at 200 mJ/cm(2) . They were then cultured within three-dimensional models of free-floating and tense collagen lattices. We analyzed collagen matrix retraction, contractile activity, the alpha-smooth muscle actin (α-SMA) cytoskeleton and the alpha2 integrin (α2 integrin) membrane receptor organization and/or expression in dermal fibroblasts. The pretreatment of fibroblasts with the extract prior to ultraviolet B irradiation showed that the cells were protected against UVB-induced suppression of α-SMA expression, fibroblast migration and contraction. These results revealed that the A. altilis heartwood extract prevents mechanical damage induced by ultraviolet B irradiation in fibroblast-embedded collagen lattices and therefore has a potential as a natural photoprotectant. This article is protected by copyright. All rights reserved.
Mots clés
Actins, metabolism, Artocarpus, chemistry, Collagen, metabolism, Female, Fibroblasts, metabolism, Humans, Integrin alpha2, metabolism, Mannose-Binding Lectins, pharmacology, Middle Aged, Plant Extracts, pharmacology, Plant Lectins, pharmacology, Radiation-Protective Agents, pharmacology, Skin, cytology, Skin Aging, drug effects, Ultraviolet Rays
Référence
Photochem. Photobiol.. 2017 May;: