Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry.
Fiche publication
Date publication
juin 2017
Journal
Journal of ovarian research
Auteurs
Membres identifiés du Cancéropôle Est :
Dr AMIOT Clotilde, Pr ROUX Christophe
Tous les auteurs :
Zver T, Mouloungui E, Berdin A, Roux C, Amiot C
Lien Pubmed
Résumé
Ovarian tissue cryopreservation is a technique for fertility preservation addressed to prepubertal girls or to patients for whom no ovarian stimulation is possible before initiation of gonadotoxic treatments. Autotransplantation of frozen-thawed ovarian tissue is the only available option for reuse but presents some limitations: ischemic tissue damages post-transplant and reintroduction of malignant cells in cases of cancer. It is therefore essential to qualify ovarian tissue before autograft on a functional and oncological point of view. Here, we aimed to isolate viable cells from human ovarian cortex in order to obtain an ovarian cell suspension analyzable by multicolor flow cytometry.
Mots clés
Cell qualification, Fertility preservation, Multicolor flow cytometry, Ovarian tissue, Tissue dissociation
Référence
J Ovarian Res. 2017 Jun;10(1):38