A toolbox of chromones and quinolones for measuring a wide range of ATP concentrations.
Fiche publication
Date publication
juillet 2017
Journal
Chemistry (Weinheim an der Bergstrasse, Germany)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves, Mr HUMBERT Nicolas
Tous les auteurs :
Pivovarenko VG, Bugera O, Humbert N, Klymchenko AS, Mély Y
Lien Pubmed
Résumé
A series of 26 3-hydroxychromones, three bis-flavonols and four 3-hydroxyquinolones were studied to evaluate their fluorescence response to interaction with ATP in buffer. The dyes differ by the total charge, the size and number of their aromatic units, as well as the position or electron donating ability of their substituents. All of them were suggested to form complexes with ATP of 1:1 and 1:2 stoichiometry, which can be evidenced by their bright fluorescence and their 3000-6000 cm-1 red-shifted excitation band. These fluorescent complexes allow detection of ATP concentrations over 3 orders of magnitude, while most other known probes cover no more than two orders. In total, the dyes allow ATP detection from 0.001 to 57 mM. In addition, most of the dye-ATP complexes can be excited in the visible and monitored in the red region of the spectrum. The response amplitude of the described dyes to ATP is as high as for the best known probes. Since complexation takes place at neutral pH, the studied dyes form a toolbox of fluorescent probes for intensiometric and ratiometric measurements of ATP concentration in a broad concentration range. Finally, the obtained results stimulate the idea that most of natural 3-hydroxyflavones in living cells may form complexes with ATP.
Mots clés
adenosine triphosphate, bis-flavonols, flavones, fluorescent probes, quinolones
Référence
Chemistry. 2017 Jul;: