The Bile Acid Nuclear Receptor FXRα Is a Critical Regulator of Mouse Germ Cell Fate.
Fiche publication
Date publication
juillet 2017
Journal
Stem cell reports
Auteurs
Membres identifiés du Cancéropôle Est :
Mme THIBAULT-CARPENTIER Christelle
Tous les auteurs :
Martinot E, Sèdes L, Baptissart M, Holota H, Rouaisnel B, Damon-Soubeyrand C, De Haze A, Saru JP, Thibault-Carpentier C, Keime C, Lobaccaro JA, Baron S, Benoit G, Caira F, Beaudoin C, Volle DH
Lien Pubmed
Résumé
Spermatogenesis is the process by which spermatozoa are generated from spermatogonia. This cell population is heterogeneous, with self-renewing spermatogonial stem cells (SSCs) and progenitor spermatogonia that will continue on a path of differentiation. Only SSCs have the ability to regenerate and sustain spermatogenesis. This makes the testis a good model to investigate stem cell biology. The Farnesoid X Receptor alpha (FXRα) was recently shown to be expressed in the testis. However, its global impact on germ cell homeostasis has not yet been studied. Here, using a phenotyping approach in Fxrα mice, we describe unexpected roles of FXRα on germ cell physiology independent of its effects on somatic cells. FXRα helps establish and maintain an undifferentiated germ cell pool and in turn influences male fertility. FXRα regulates the expression of several pluripotency factors. Among these, in vitro approaches show that FXRα controls the expression of the pluripotency marker Lin28 in the germ cells.
Mots clés
Aging, Animals, Cells, Cultured, Female, Fertility, Gene Deletion, Gene Expression Regulation, Leydig Cells, cytology, Male, Mice, Inbred C57BL, RNA-Binding Proteins, genetics, Receptors, Cytoplasmic and Nuclear, genetics, Reproduction, Sertoli Cells, cytology, Spermatogenesis, Spermatozoa, cytology, Testis, cytology
Référence
Stem Cell Reports. 2017 Jul 11;9(1):315-328