Morphine Binds Creatine Kinase B and Inhibits Its Activity.
Fiche publication
Date publication
janvier 2018
Journal
Frontiers in cellular neuroscience
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CIANFERANI Sarah, Dr GOUMON Yannick
Tous les auteurs :
Weinsanto I, Mouheiche J, Laux-Biehlmann A, Delalande F, Marquette A, Chavant V, Gabel F, Cianferani S, Charlet A, Parat MO, Goumon Y
Lien Pubmed
Résumé
Morphine is an analgesic alkaloid used to relieve severe pain, and irreversible binding of morphine to specific unknown proteins has been previously observed. In the brain, changes in the expression of energy metabolism enzymes contribute to behavioral abnormalities during chronic morphine treatment. Creatine kinase B (CK-B) is a key enzyme involved in brain energy metabolism. CK-B also corresponds to the imidazoline-binding protein I which binds dopamine (a precursor of morphine biosynthesis) irreversibly. Using biochemical approaches, we show that recombinant mouse CK-B possesses a μM affinity for morphine and binds to morphine . The complex formed by CK-B and morphine is resistant to detergents, reducing agents, heat treatment and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). CK-B-derived peptides CK-B and CK-B were identified as two specific morphine binding-peptides. , morphine (1-100 μM) significantly reduces recombinant CK-B enzymatic activity. Accordingly, morphine administration (7.5 mg/kg, i.p.) to mice significantly decreased brain extract CK-B activity compared to saline-treated animals. Together, these results show that morphine strongly binds CK-B and inhibits its activity and .
Mots clés
complex, creatine kinase, high affinity, ligand-binding protein, morphine
Référence
Front Cell Neurosci. 2018 ;12:464