The contribution of hydrogen peroxide to the radiosensitizing effect of gold nanoparticles.
Fiche publication
Date publication
décembre 2018
Journal
Colloids and surfaces. B, Biointerfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Pr FROMM Michel, Pr ROUX Stéphane
Tous les auteurs :
Khalil TT, Bazzi R, Roux S, Fromm M
Lien Pubmed
Résumé
Plasmid DNA in aerated aqueous solution is used as a probe to determine whose of the reactive oxygen species (ROS) generated after absorption of ultra-soft X-rays (USX) take part in biomolecule damage in the presence and in absence of Gold Nano-Particles (GNP) and specific scavengers. Citrate-coated GNPs with core sizes of 6, 10 and 25 nm are synthetized and characterized, especially in terms of plasmon band shift, ζ-potential and hydrodynamic radii (respectively 9, 21 and 30 nm). We confirm the radiosensitizing effect of GNP and show that the SSB number per plasmid increases when, for a same mass of gold element, the core size of the gold nanoparticles decreases. Hydroxyl radicals (OH) are scavenged using the positively-charged 2-amino-2-hydroxymethyl-1,3-propanediol (TRIS) and the neutral dimethyl sulfoxide (DMSO) molecules. Due to both negatively-charged environments of DNA and GNP, at identical scavenging capacity, TRIS is more effective at quenching OH than DMSO. The strong radiosensitizing effect of hydroxyl radicals is confirmed. Methanoate anions are then used to transform OH into hydrogen peroxide; the latter being known to be non-aggressive regarding DNA in the absence of easily oxidable metallic ions (Fenton reactions). Surprisingly, in the presence of GNP, high DNA damage yields are observed even though hydrogen peroxide might not be hold as responsible. Conversely, the radiosensitizing effect of GNP is not observed anymore when HO is scavenged using pyruvate ions. We demonstrate that hydrogen peroxide constitutes quite unexpectedly a hidden stock of OH which are activated at the surface of the GNP by decomposition of HO molecules.
Mots clés
Gold nanoparticles, Hydrogen peroxide, Plasmid DNA, ROS scavenger, Ultra-soft X-rays
Référence
Colloids Surf B Biointerfaces. 2018 Dec 17;175:606-613