Polymer analysis in the second dimension: preliminary studies for the characterisation of polymers with 2D MS.
Fiche publication
Date publication
août 2017
Journal
Analytical chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DELSUC Marc-André
Tous les auteurs :
Floris F, Vallotto C, Chiron L, Lynch A, Barrow MP, Delsuc MA, O'Connor PB
Lien Pubmed
Résumé
Two-Dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FT-ICR MS or 2D MS) allows direct correlation between precursor and fragment ions without isolation prior to fragmentation. The method has been optimised for the analysis of complex mixtures, and used so far for the analysis of small molecules, peptides obtained by tryptic digestion of proteins, and entire proteins. In this work, a 2D MS method is developed to characterise complex mixtures of polymers using IRMPD and ECD as fragmentation techniques, and TPGS, Polysorbate 80, and PMMA as analytes. The use of 2D MS allowed generation of fragment m/z values for all the compounds in the mixture at once, and allowed tandem mass spectrometry of species very close in m/z, that would have been difficult to isolate with a quadrupole for standard MS/MS. Furthermore, the use of unique features of 2D MS such as the extraction of neutral-loss lines allowed the successful assignment of peaks from low abundant species that would have been more difficult with standard MS/MS. For all the samples, the amount of information obtained with 2D MS was comparable with what obtained with multiple 1D MS/MS experiments targeted on each individual component within each mixture but required a single experiment of about 20 to 40 minutes.
Référence
Anal. Chem.. 2017 Aug;: