Non-Uniform Sampling (NUS) Acquisition of two-dimensional (2D) FT-ICR MS for increased mass resolution of MS/MS precursor ions.
Fiche publication
Date publication
août 2017
Journal
Analytical chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DELSUC Marc-André
Tous les auteurs :
Bray F, Bouclon J, Chiron L, Witt M, Delsuc MA, Rolando C
Lien Pubmed
Résumé
Obtaining the full MS/MS map for fragments and precursors of complex mixtures without hyphenation with chromatographic sepa-ration by a data-independent acquisition is a challenge in mass spec-trometry which is solved by two-dimensional (2D) Fourier trans-form ion cyclotron resonance mass spectrometry (FT-ICR MS). Until now 2D FT-ICR MS afforded only a moderate resolution for precursor ion since this resolution is limited by the number of evo-lution interval steps to which the number of scans, the acquisition time and the sample consumption are proportional. An overnight acquisition is already required to reach a quadrupole mass filter-like unit mass resolution. Here, we report that 2D FT-ICR MS using non-uniform sampling (NUS) obtained by randomly skipping points in the first dimension corresponding to the precursor selec-tion gives access, after data processing, to the same structural in-formation contained in a complex mixture. The resolution increases roughly as the inverse of the NUS ratio, up to 26 times at NUS 1/32, leading to an acquisition time reduced in the same ratio com-pared to a classical acquisition at the same resolution. As an exam-ple, the analysis of a natural oil is presented.
Référence
Anal. Chem.. 2017 Aug;: