Establishing contact between cell-laden hydrogels and metallic implants with a biomimetic adhesive for cell therapy supported implants.
Fiche publication
Date publication
août 2017
Journal
Biomedical materials (Bristol, England)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LAVALLE Philippe
Tous les auteurs :
Barthes J, Mutschler A, Dollinger C, Gaudinat G, Lavalle P, Le Houerou V, McGuinness GB, Vrana NE
Lien Pubmed
Résumé
For in-dwelling implants, controlling the biological interface is a crucial parameter to promote tissue regeneration and prevent implant failure. For this purpose, one possibility is to facilitate the establishment of the interface with cell-laden hydrogel fixed to the implant. However, for proper functioning, the stability of the hydrogel on the implant should be ensured. Modification of implant surfaces with an adhesive represents a promising strategy to promote the adhesion of cell-laden hydrogel on the implant. Herein, we developed a peptidic adhesive based on mussel foot protein (L-DOPA-L-Lysine)2-L-DOPA that can be applied directly on the surface of an implant. At physiological pH, unoxidized (L-DOPA-L-Lysine)2-L-DOPA expected to strongly adhere on metal/metal oxide surface formed only very thin coatings. Once oxidized at physiological pH, (L-DOPA-L-Lysine)2-L-DOPA forms an adhesive coating about 20 nanometers thick. In oxidized conditions, L-Lysine can adhere to metallic substrate via electrostatic interaction. Oxidized L-DOPA allows to form a coating through self-polymerization and can react with amines so this adhesive can be used to fix ECM based materials on implant surfaces through the reaction of quinones with amino groups. Hence, a stable interface between a soft gelatin hydrogel and metallic surfaces was achieved and the strength of adhesion was investigated. We have shown that the adhesive is non-cytotoxic to encapsulated cells and enabled the adhesion of gelatin soft hydrogels for 21 days on metallic substrates in liquid conditions. The adhesion properties of this anchoring peptide was quantified by a 180° peeling test. Utilization of a biomimetic adhesive interface is an important tool for the application of cell-laden hydrogels to metallic implant surfaces as hydrogel/implant interface can be ensured without relying on the properties of the deposited biomaterials.
Mots clés
3T3 Cells, Adhesives, Animals, Biocompatible Materials, chemistry, Biomimetic Materials, chemistry, Bivalvia, Cell Adhesion, Dihydroxyphenylalanine, chemistry, Fibroblasts, chemistry, Gelatin, chemistry, Humans, Hydrogels, chemistry, Hydrogen-Ion Concentration, Lysine, chemistry, Metals, chemistry, Mice, Peptides, chemistry, Prostheses and Implants, Prosthesis Design, methods, Quinones, chemistry, Transglutaminases, chemistry
Référence
Biomed Mater. 2017 Aug;: