Direct comparison of UDP-glucuronosyltransferase and cytochrome P450 activities in human liver microsomes, plated and suspended primary human hepatocytes from five liver donors.
Fiche publication
Date publication
août 2017
Journal
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BACHELLIER Philippe
Tous les auteurs :
den Braver-Sewradj SP, den Braver MW, Baze A, Decorde J, Fonsi M, Bachellier P, Vermeulen NPE, Commandeur JNM, Richert L, Chris Vos J
Lien Pubmed
Résumé
UDP-glucuronosyltransferases (UGTs) and cytochrome P450s (CYPs) are the major enzymes involved in hepatic metabolism of drugs. Hepatic drug metabolism is commonly investigated using human liver microsomes (HLM) or primary human hepatocytes (PHH). We describe the development of a sensitive assay to phenotype activities of six major hepatic UGT isoforms (UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A9 and UGT2B7) in intact PHH by analysis of glucuronidation of selective probe substrates. The non-selective, general substrate 7-hydroxycoumarin was included for comparison. For each liver donor preparation (five donors) UGT activities in cryopreserved suspended and plated PHH were compared to HLM prepared from the same donors. Standard CYP reaction phenotyping of seven major isoforms was performed in parallel. For all donors, CYP- and UGT-isoforms activity profiles were comparable in PHH and HLM, indicating that reaction phenotyping with selective probe substrates in intact cells primarily reflects respective CYP or UGT activity. System-dependent effects on UGT and CYP isoform activity were still found. While UGT activity of UGT1A1 was equivalent in plated and suspended PHH, UGT1A3, UGT1A6 and UGT2B7 activity was higher in suspended PHH and UGT1A9 and UGT1A4 activity was higher in plated PHH. The well-known decrease in activity of most CYP isoforms in plated compared to suspended PHH was confirmed. Importantly, we found a significant loss in CYP2C19 and CYP2B6 in HLM, activity being lower than in intact cells. Taken together, these findings implicate that, dependent on the UGT or CYP isoforms involved in the metabolism of a given compound, the outcome of metabolic assays is strongly dependent on the choice of the in vitro system. The currently described UGT- and CYP- activity profiling method can be used as a standard assay in intact cells and can especially aid in reaction phenotyping of in vitro systems for which a limited number of cells are available.
Mots clés
4-hydroxyindole (PubChem CID:75421), 7-hydroxcoumarin (PubChem CID:5281426), Bufuralol (PubChem CID:71733), Bupropion (PubChem CID:444), Chenodeoxycholic acid (PubChem CID:10133), Coumarin (PubChem CID:323), Cytochrome P450, Diclofenac (PubChem CID:3033), Human liver microsomes, Mephenytoin (PubChem CID:4060), Midazolam (PubChem CID:4192), Naloxone (PubChem CID:5284696), Phenacetin (PubChem CID:4754), Primary human hepatocytes, Propofol (PubChem CID:4943), Reaction phenotyping, Trifluoperazine (PubChem CID:5566), UDP-glucuronosyltransferases, β-estradiol (PubChem CID:5757)
Référence
Eur J Pharm Sci. 2017 Aug;: