Two functionally redundant sources of retinoic acid secure spermatogonia differentiation in the seminiferous epithelium.
Fiche publication
Date publication
novembre 2018
Journal
Development (Cambridge, England)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr TELETIN Marius, Dr MARK Manuel
Tous les auteurs :
Teletin M, Vernet N, Yu J, Klopfenstein M, Jones JW, Féret B, Kane MA, Ghyselinck NB, Mark M
Lien Pubmed
Résumé
In mammals, all- retinoic acid (ATRA) is instrumental to spermatogenesis. It is synthesized by two retinaldehyde dehydrogenases (RALDH) present in both Sertoli cells (SC) and germ cells (GC). In order to determine the relative contributions of each source of ATRA, we have generated mice lacking all RALDH activities in the seminiferous epithelium (SE). We show that both the SC- and GC-derived sources of ATRA cooperate to initiate and propagate spermatogenetic waves at puberty. In adults, they exert redundant functions and, against all expectations, the GC-derived source does not perform any specific role despite contributing to two-third of the total amount of ATRA present in the testis. The production from SC is sufficient to maintain the periodic expression of genes in SC, as well and the cycle and wave of the SE which account for the steady production of spermatozoa. The production from SC is also specifically required for spermiation. Importantly, our study shows that spermatogonia differentiation depends upon the ATRA synthesized by RALDH inside the SE, while initiation of meiosis and expression of STRA8 by spermatocytes can occur without ATRA.
Mots clés
Aldh8a1 knockout, Immunohistochemistry, Lgals1, Puberty, Spermatogenesis
Référence
Development. 2018 Nov 28;: