Substitution of strontium and boron into hydroxyapatite crystals: Effect on physicochemical properties and biocompatibility with human Wharton-Jelly stem cells.
Fiche publication
Date publication
octobre 2017
Journal
Materials science & engineering. C, Materials for biological applications
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr VELARD Frédéric, Dr KERDJOUDJ Halima
Tous les auteurs :
Kolmas J, Velard F, Jaguszewska A, Lemaire F, Kerdjoudj H, Gangloff SC, Kaflak A
Lien Pubmed
Résumé
Hydroxyapatite (HA) enriched with strontium and boron ions was synthesized using two different methods: the precipitation method (Sr,B-HAw) and the dry method (Sr,B-HAd). Additionally, for the sake of comparison, the "pure" unsubstituted HA was prepared together with HAs substituted only with one type of a foreign ion. The obtained materials were subjected to physicochemical analysis with the use of various analytical methods, such as powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), inductively coupled plasma optical emission spectroscopy (ICP-OES), Fourier transform infrared spectroscopy (FT-IR) and solid-state proton nuclear magnetic resonance (H ssNMR). All the obtained materials were also biologically tested for their potential cytotoxicity. The obtained materials (Sr,B-HAw and Sr,B-HAd) were homogeneous and respectively showed nano- and microcrystal apatitic structures. The simultaneous introduction of Sr and BO ions turned out to be more effective in respect of the dry method. Of importance, doped materials obtained using both synthesis routes have been demonstrated to be biocompatible, opening the way for medical applications.
Mots clés
Boron, Durapatite, Humans, Spectroscopy, Fourier Transform Infrared, Stem Cells, Strontium, X-Ray Diffraction
Référence
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:638-646