Protein Kinase A Deregulation in the Medial Prefrontal Cortex Impairs Working Memory in Murine Oligophrenin-1 Deficiency.
Fiche publication
Date publication
novembre 2017
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHELLY Jamel
Tous les auteurs :
Zhang CL, Aime M, Laheranne E, Houbaert X, El Oussini H, Martin C, Lepleux M, Normand E, Chelly J, Herzog E, Billuart P, Humeau Y
Lien Pubmed
Résumé
Classical and systems genetics have identified wide networks of genes associated with cognitive and neurodevelopmental diseases. In parallel to deciphering the role of each of these genes in neuronal or synaptic function, evaluating the response of neuronal and molecular networks to gene loss of function could reveal some pathophysiological mechanisms potentially accessible to nongenetic therapies. Loss of function of the Rho-GAP oligophrenin-1 is associated with cognitive impairments in both human and mouse. Upregulation of both PKA and ROCK has been reported in mice, but it remains unclear whether kinase hyperactivity contributes to the behavioral phenotypes. In this study, we thoroughly characterized a prominent perseveration phenotype displayed by -deficient mice using a Y-maze spatial working memory (SWM) test. We report that deficiency in the mouse generated severe cognitive impairments, characterized by both a high occurrence of perseverative behaviors and a lack of deliberation during the SWM test. and pharmacological experiments suggest that PKA dysregulation in the mPFC underlies cognitive dysfunction in -deficient mice, as assessed using a delayed spatial alternation task results. Functionally, mPFC neuronal networks appeared to be affected in a PKA-dependent manner, whereas hippocampal-PFC projections involved in SWM were not affected in mice. Thus, we propose that discrete gene mutations in intellectual disability might generate "secondary" pathophysiological mechanisms, which are prone to become pharmacological targets for curative strategies in adult patients. Here we report that deficiency generates severe impairments in performance at spatial working memory tests, characterized by a high occurrence of perseverative behaviors and a lack of decision making. This cognitive deficit is consecutive to PKA deregulation in the mPFC that prevents KO mice to exploit a correctly acquired rule. Functionally, mPFC neuronal networks appear to be affected in a PKA-dependent manner, whereas behaviorally important hippocampal projections were preserved by the mutation. Thus, we propose that discrete gene mutations in intellectual disability can generate "secondary" pathophysiological mechanisms prone to become pharmacological targets for curative strategies in adults.
Mots clés
Animals, Cyclic AMP-Dependent Protein Kinases, metabolism, Cytoskeletal Proteins, deficiency, GTPase-Activating Proteins, deficiency, Male, Maze Learning, physiology, Memory Disorders, metabolism, Memory, Short-Term, physiology, Mice, Mice, Inbred C57BL, Mice, Knockout, Nerve Net, metabolism, Nuclear Proteins, deficiency, Organ Culture Techniques, Prefrontal Cortex, metabolism, Random Allocation
Référence
J. Neurosci.. 2017 Nov 15;37(46):11114-11126