Increased IL-22- and IL-17A-Producing Mucosal-Associated Invariant T Cells in the Peripheral Blood of Patients With Ankylosing Spondylitis.
Fiche publication
Date publication
janvier 2018
Journal
Frontiers in immunology
Auteurs
Membres identifiés du Cancéropôle Est :
Mme LAHEURTE Caroline, Pr TOUSSIROT Eric
Tous les auteurs :
Toussirot É, Laheurte C, Gaugler B, Gabriel D, Saas P
Lien Pubmed
Résumé
The IL-23/T helper 17 (Th17) axis plays an important role in joint inflammation in ankylosing spondylitis (AS). Conventional CD4 Th17 cells are a major source of IL-17A. IL-22 is another cytokine implicated in AS pathophysiology and is produced by Th17 and Th22 cells. In this study, we aimed to analyze conventional and non-conventional T cell subsets producing IL-17A and IL-22 in patients with AS. We thus evaluated the intracellular staining for IL-17A, IL-22, and IFN-γ in peripheral blood mononuclear cells of 36 patients with AS and 55 age- and sex-matched healthy controls (HC). Conventional CD4 and CD8 T cells, γδ T cells, and mucosal-associated invariant T (MAIT) cells were evaluated. In patients with AS, we found a decreased frequency and number of γδ T cells, of MAIT cells and of IFN-γ CD4 and CD8 T cells. Th17-related IL-17A/IFN-γ CD4 T cells were decreased in AS. The number of IL-22 MAIT cells was higher in AS compared with HC, as well as the number of IFN-γ/IL-17A MAIT cells. The number of IFN-γ/IL-17A MAIT cells was higher only in female patients with AS compared with female HC. The cellular source of IL-17A was thus not restricted to conventional Th17 CD4 T cells and might involve innate-like T cells, such as MAIT cells. Circulating MAIT cells producing IL-22 were increased in AS. These results strengthen the importance of innate and innate-like sources of IL-17A and/or IL-22.
Mots clés
IL-17A, IL-22, ankylosing spondylitis, mucosal immunity, mucosal-associated invariant T cells
Référence
Front Immunol. 2018 ;9:1610