FRET-SLiM on native autofluorescence: a fast and reliable method to study interactions between fluorescent probes and lignin in plant cell wall.
Fiche publication
Date publication
janvier 2018
Journal
Plant methods
Auteurs
Membres identifiés du Cancéropôle Est :
Dr TERRYN Christine
Tous les auteurs :
Terryn C, Paës G, Spriet C
Lien Pubmed
Résumé
Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that necessitates some pretreatments and several types of catalysts to be transformed efficiently. In particular, enzymes degrading lignocellulose can become inactivated due to their binding to lignin through non-specific interactions, leading to a loss in catalytic efficiency of industrial processes. Gaining more knowledge in the strength of interactions would allow optimizing enzymes and selecting appropriate pretreatments.
Mots clés
Autofluorescence, FRET, Fluorescence lifetime, Interaction, Lignocellulose, SLiM
Référence
Plant Methods. 2018 ;14:74