A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications.
Fiche publication
Date publication
janvier 2018
Journal
Journal of ovarian research
Auteurs
Membres identifiés du Cancéropôle Est :
Dr AMIOT Clotilde, Pr ROUX Christophe
Tous les auteurs :
Mouloungui E, Zver T, Roux C, Amiot C
Lien Pubmed
Résumé
Autotransplantation of cryopreserved ovarian cortex can be associated with a risk of cancer cell reseeding. This issue could be eliminated by grafting isolated preantral follicles. Collagenase NB6 is an enzyme produced under good manufacturing practices (GMP) in compliance with requirements for tissue engineering and transplantation in humans and thus can be used to isolate preantral follicles from ovarian tissue in the framework of further clinical applications. Multicolor flow cytometry is an effective tool to evaluate the potential contamination of follicular suspensions by leukemic cells.
Mots clés
Collagenase NB6, Good manufacturing practices, Human follicle isolation, Leukemic cell purification
Référence
J Ovarian Res. 2018 Jan 5;11(1):4