Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal.
Fiche publication
Date publication
mars 2018
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LEFEBVRE Olivier
Tous les auteurs :
Rayagiri SS, Ranaldi D, Raven A, Mohamad Azhar NIF, Lefebvre O, Zammit PS, Borycki AG
Lien Pubmed
Résumé
A central question in stem cell biology is the relationship between stem cells and their niche. Although previous reports have uncovered how signaling molecules released by niche cells support stem cell function, the role of the extra-cellular matrix (ECM) within the niche is unclear. Here, we show that upon activation, skeletal muscle stem cells (satellite cells) induce local remodeling of the ECM and the deposition of laminin-α1 and laminin-α5 into the basal lamina of the satellite cell niche. Genetic ablation of laminin-α1, disruption of integrin-α6 signaling or blocking matrix metalloproteinase activity impairs satellite cell expansion and self-renewal. Collectively, our findings establish that remodeling of the ECM is an integral process of stem cell activity to support propagation and self-renewal, and may explain the effect laminin-α1-containing supports have on embryonic and adult stem cells, as well as the regenerative activity of exogenous laminin-111 therapy.
Mots clés
Animals, Basement Membrane, cytology, Cell Differentiation, physiology, Cell Self Renewal, physiology, Extracellular Matrix, metabolism, Humans, Mice, Inbred C57BL, Muscle Fibers, Skeletal, cytology, Muscle, Skeletal, cytology, Satellite Cells, Skeletal Muscle, cytology, Signal Transduction, Stem Cell Niche, physiology
Référence
Nat Commun. 2018 03 14;9(1):1075