The NC domain of HIV-1 Gag contributes to the interaction of Gag with TSG101.
Fiche publication
Date publication
mars 2018
Journal
Biochimica et biophysica acta
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves, Dr RICHERT Ludovic
Tous les auteurs :
El Meshri SE, Boutant E, Mouhand A, Thomas A, Larue V, Richert L, Vivet-Boudou V, Mély Y, Tisné C, Muriaux D, de Rocquigny H
Lien Pubmed
Résumé
HIV-1 Gag polyprotein orchestrates the assembly of viral particles. Its C-terminus consists of the nucleocapsid (NC) domain that interacts with RNA, and the p6 domain containing the PTAP motif that binds the cellular ESCRT factor TSG101 and ALIX. Deletion of the NC domain of Gag (GagNC) results in defective Gag assembly, a decrease in virus production and, thus probably affects recruitment of the ESCRT machinery. To investigate the role of GagNC in this recruitment, we analysed its impact on TSG101 and ALIX localisations and interactions in cells expressing Gag.
Mots clés
FRET, Gag, HIV, NMR, Nucleocapsid, TSG101
Référence
Biochim. Biophys. Acta. 2018 Mar 20;1862(6):1421-1431