Antibody directed against mannan of the Mycobacterium tuberculosis cell envelope provokes blood-brain barrier breakdown.
Fiche publication
Date publication
février 2000
Journal
Journal of neuroimmunology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr NAMER Izzie-Jacques
Tous les auteurs :
Namer IJ, Steibel J
Lien Pubmed
Résumé
Previous studies demonstrated that blood-brain barrier (BBB) breakdown observed at the cerebral level during experimental allergic encephalomyelitis (EAE) arises from the presence of Mycobacterium tuberculosis in Freund's adjuvant and not only from the encephalitogenic antigens. The main objective of this study was to check if the mannan moiety of lipoarabinomannan present in the mycobacterial cell envelope is responsible for an immune response provoking a BBB breakdown. The results showed that: firstly, the complete Freund's adjuvant (CFA) contains a high release of polysaccharides; secondly, the rats immunized with the CFA present important serum concentration of anti-mannan antibody; and finally, a single 2-mg dose of anti-mannan antibody injected intravenously in naive rats provokes an immediate and reversible BBB breakdown. These results suggest that mannan arising from the solubilization of the mycobacterial cell wall in Freund's adjuvant induces a high production of anti-mannan antibody, which, in turn, provokes a BBB breakdown and possibly facilitates the induction of EAE.
Mots clés
Animals, Antibodies, Bacterial, pharmacology, Blood-Brain Barrier, Cell Membrane, immunology, Encephalomyelitis, Autoimmune, Experimental, etiology, Female, Freund's Adjuvant, immunology, Mannans, immunology, Mice, Mice, Inbred BALB C, Mycobacterium tuberculosis, immunology, Polysaccharides, Bacterial, analysis, Rats, Rats, Inbred Lew
Référence
J. Neuroimmunol.. 2000 Feb;103(1):63-8