Development of a quantitative PCR detecting Cunninghamella bertholletiae to help in diagnosing this rare and aggressive mucormycosis.
Fiche publication
Date publication
avril 2018
Journal
Bone marrow transplantation
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BERCEANU Ana, Pr DECONINCK Eric
Tous les auteurs :
Bellanger AP, Berceanu A, Rocchi S, Valot B, Fontan J, Chauchet A, Belin N, Scherer E, Deconinck E, Navellou JC, Millon L
Lien Pubmed
Résumé
Mucormycosis is an invasive mold infection, frequently fatal in immunocompromised patients. We report the case of a patient with chronic lymphocytic leukemia admitted to the hematology unit for febrile aplasia. Pulmonary lesions suggesting a fungal infection expanded/increased despite a combination of posaconazole and liposomal amphotericin B. The fungal biomarkers performed repeatedly were negative. At D65 after chemotherapy a bronchial biopsy was positive for Cunninghamella bertholletiae. The patient died despite appropriate antifungal management. A qPCR targeting Cunninghamella was developed a posteriori, and a retrospective analysis showed that a sample was positive more than 30 days before culture-based identification could be made.
Référence
Bone Marrow Transplant.. 2018 Apr 30;: