Cell migration and proliferation are not discriminatory factors in the in vitro sociologic behavior of bronchial epithelial cell lines.
Fiche publication
Date publication
septembre 2002
Journal
Cell motility and the cytoskeleton
Auteurs
Membres identifiés du Cancéropôle Est :
Pr POLETTE Myriam
Tous les auteurs :
Matos M, Raby BN, Zahm JM, Polette M, Birembaut P, Bonnet N
Lien Pubmed
Résumé
A model of cellular cohesion has been developed, which permits the in vitro study of the spatial and temporal distribution of two human bronchial cell lines. The spatial distribution of cells in culture was characterized from videomicroscopic recordings and analyzed using an algorithmic program of cellular sociology based on the use of three geometrical models: Voronoï's partition, Delaunay's graph, the and minimum spanning tree (MST). The results obtained suggested that the manner of cellular cohesiveness could be used to differentiate between the organizational behaviors of the cell lines: non-invasive 16HBE14o- cells rapidly formed clusters with a cohesive organization, whereas invasive BZR cells remained isolated and were characterized by a non-cohesive organization. Videomicroscopic and image analysis techniques also demonstrated that cell migration and proliferation are not discriminatory factors for explaining differences in the spatial organizations of the two cell lines. We concluded that the random nature of cell movement combined with the cell adhesion capacity are determinant factors in cell cluster formation.
Mots clés
Cell Adhesion, physiology, Cell Aggregation, physiology, Cell Communication, physiology, Cell Division, physiology, Cell Line, Transformed, Cell Movement, physiology, Humans, Image Processing, Computer-Assisted, Microscopy, Video, Models, Biological, Neoplasm Invasiveness, physiopathology, Neoplasm Metastasis, physiopathology, Respiratory Mucosa, cytology, Time Factors
Référence
Cell Motil. Cytoskeleton. 2002 Sep;53(1):53-65