PROBE: analysis and visualization of protein block-level evolution.
Fiche publication
Date publication
mai 2018
Journal
Bioinformatics (Oxford, England)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr POCH Olivier, Dr THOMPSON Julie
Tous les auteurs :
Kress A, Lecompte O, Poch O, Thompson JD
Lien Pubmed
Résumé
Comparative studies of protein sequences are widely used in evolutionary and comparative genomics studies, but there is a lack of efficient tools to identify conserved regions ab initio within a protein multiple alignment. PROBE provides a fully automatic analysis of protein family conservation, to identify conserved regions, or 'blocks', that may correspond to structural / functional domains or motifs. Conserved blocks are identified at two different levels: (i) family level blocks indicate sites that are probably of central importance to the protein's structure or function, and (ii) sub-family level blocks highlight regions that may signify functional specialization, such as binding partners, etc. All conserved blocks are mapped onto a phylogenetic tree and can also be visualized in the context of the multiple sequence alignment. PROBE thus facilitates in-depth studies of sequence-structure-function-evolution relationships, and opens the way to block-level phylogenetic profiling.
Référence
Bioinformatics. 2018 May 7;: