In vitro interaction and biocompatibility of titanate nanotubes with microglial cells.
Fiche publication
Date publication
juin 2018
Journal
Toxicology and applied pharmacology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LIZARD Gérard, Pr MILLOT Nadine, Dr BOUDON Julien
Tous les auteurs :
Sruthi S, Loiseau A, Boudon J, Sallem F, Maurizi L, Mohanan PV, Lizard G, Millot N
Lien Pubmed
Résumé
Titanate nanotubes (TiONts) are promising agents for biomedical applications. Microglial activation and associated oxidative burst are major challenges in drug delivery applications across the brain. Here, TiONts were designed for drug delivery systems by functionalizing them with (3-aminopropyl) triethoxysilane (APTES), their interactions and biocompatibility were studied in vitro using murine microglial BV-2 cells. TiONts-APTES exposure resulted in increased ROS production and transient mitochondrial hyperpolarization. However, there was no indication of microglial proliferation in BV-2 cells as suggested by cell cycle analysis and cell count. The internalization process of TiONts-APTES into cells by endocytosis vesicles and passive diffusion were proved by transmission electron microscopy (TEM) with and without amiloride, the endocytosis inhibiting agent. In addition, the TiONts-APTES exhibited good biocompatibility on microglial BV-2 cells as revealed by the morphology and viability analysis.
Mots clés
Apoptosis, Lysosomal integrity, Microglial activation, Mitochondrial hyperpolarisation, Reactive oxygen species, Titanate nanotubes
Référence
Toxicol. Appl. Pharmacol.. 2018 Jun 13;: