Virus preparations from the mixed-infected P70 Pinot Noir accession exhibit GLRaV-1/GVA 'end-to-end' particles.
Fiche publication
Date publication
août 2018
Journal
Archives of virology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ERHARDT Mathieu
Tous les auteurs :
Alliaume A, Reinbold C, Erhardt M, Beuve M, Hily JM, Lemaire O, Herrbach E
Lien Pubmed
Résumé
P70 is a Pinot Noir grapevine accession that displays strong leafroll disease symptoms. A high-throughput sequencing (HTS)-based analysis established that P70 was mixed-infected by two variants of grapevine leafroll-associated virus 1 (GLRaV-1, genus Ampelovirus) and one of grapevine virus A (GVA, genus Vitivirus) as well as by two viroids (hop stunt viroid [HSVd] and grapevine yellow speckle viroid 1 [GYSVd1]) and four variants of grapevine rupestris stem pitting-associated virus (GRSPaV). Immunogold labelling using gold particles of two different diameters revealed the existence of 'hybrid' particles labelled at one end as GLRaV-1, with the rest labelled as GVA. In this work, we suggest that immunogold labelling can provide information about the biology of the viruses, going deeper than just genomic information provided by HTS, from which no recombinant or 'chimeric' GLRaV-1/GVA sequences had been identified in the dataset. Our observations suggest an unknown interaction between members of two different viral species that are often encountered together in a single grapevine, highlighting potential consequences in the vector biology and epidemiology of leafroll and rugose-wood diseases.
Mots clés
Closteroviridae, classification, Plant Diseases, virology, Recombination, Genetic, Viroids, classification, Virus Cultivation, Vitis, virology
Référence
Arch. Virol.. 2018 Aug 16;: