The UPF1 interactome reveals interaction networks between RNA degradation and translation repression factors in Arabidopsis.
Fiche publication
Date publication
juillet 2018
Journal
The Plant journal : for cell and molecular biology
Auteurs
Membres identifiés du Cancéropôle Est :
Mr HAMMANN Philippe, Dr MUTTERER Jérôme
Tous les auteurs :
Chicois C, Scheer H, Garcia S, Zuber H, Mutterer J, Chicher J, Hammann P, Gagliardi D, Garcia D
Lien Pubmed
Résumé
The RNA-helicase UPF1 is a key factor of Nonsense-Mediated Decay (NMD), an mRNA decay pathway involved in RNA quality control and in the fine-tuning of gene expression. UPF1 recruits UPF2 and UPF3 to constitute the NMD core complex, which is conserved across eukaryotes. No other components of UPF1-containing RNPs are known in plants, despite its key role in regulating gene expression. Here, we report the identification of a large set of proteins that copurify with the Arabidopsis UPF1, either in a RNA-dependent or RNA-independent manner. We found that like UPF1, several of its copurifying proteins have a dual localization in the cytosol and in P-bodies, which are dynamic structures formed by the condensation of translationally repressed mRNPs. Interestingly, more than half of the proteins of the UPF1 interactome also copurify with DCP5, a conserved translation repressor also involved in P-body formation. We identified a terminal nucleotidyltransferase, ribonucleases and several RNA helicases among the most significantly enriched proteins copurifying with both UPF1 and DCP5. Among these RNA helicases are the homologs of DDX6/Dhh1, known as translation repressors in humans and yeast, respectively. Overall, this study reports a large set of proteins associated with the Arabidopsis UPF1 and DCP5, two components of P-bodies, and reveals an extensive interaction network between RNA degradation and translation repression factors. Using this resource, we identified five hitherto unknown components of P-bodies in plants, pointing out the value of this dataset for the identification of proteins potentially involved in translation repression and/or RNA degradation. This article is protected by copyright. All rights reserved.
Mots clés
DCP5, DDX6, P-bodies, RNA degradation, RNA helicase, UPF1, mass spectrometry, nonsense-mediated decay, proteome, translation repression
Référence
Plant J.. 2018 Jul 7;: