Induction of syngeneic intradermal and orthotopic epidermal carcinomas in hairless Skh-1 mice as a reproducible model for experiments.
Fiche publication
Journal
Anticancer research
Auteurs
Membres identifiés du Cancéropôle Est :
Dr VIGNERON Céline
Tous les auteurs :
Christophe M, Bisson JF, Notter D, Parache RM, Vigneron C, Guillemin F
Lien Pubmed
Résumé
Epidermoid carcinomas, clinically and histologically similar to human squamous cell carcinomas (SCC), were obtained in hairless Skh-1 mice. Tumor cells originated from chemically-induced skin cancers. We developed three models of orthotopic skin tumors: (1) intradermal injection of a tumor cell suspension, (2) superficial abrasion of the skin, cell grafting and application of a hydrocolloid dressing, (3) skin incision, seeding and application of a hydrocolloid dressing. Intradermal injection was 100% successful. Skin incision, displaying histological evidence of rapid invasive tumor growth, was 75% successful. Though skin tumor growth after abrasion was only 20% successful, the tumor histogenesis exactly imitated human SCC development. These carcinomas provide research models for further experiments such as photodynamic therapy or antiangiogenesis therapy.
Mots clés
Animals, Carcinoma, Squamous Cell, chemically induced, Disease Models, Animal, Female, Injections, Intradermal, Mice, Mice, Hairless, Neoplasm Transplantation, methods, Reproducibility of Results, Skin Neoplasms, chemically induced
Référence
Anticancer Res.. ;22(5):2869-75