Bio-specific and bio-orthogonal chemistries to switch-off the quencher of a FRET-based fluorescent probe: application to living-cell biothiol imaging.
Fiche publication
Date publication
septembre 2014
Auteurs
Membres identifiés du Cancéropôle Est :
Dr WAGNER Alain
Tous les auteurs :
Egloff C, Jacques SA, Nothisen M, Weltin D, Calligaro C, Mosser M, Remy JS, Wagner A
Lien Pubmed
Résumé
We report the first molecular system that is responsive to both a bio-specific and a bio-orthogonal stimulus. This dual activation process was applied to the design of a biothiol-specific FRET-based fluorescent probe that could be turned-on via an original concept of quencher bleaching.
Référence
Chem Commun (Camb). 2014 Sep 11;50(70):10049-51