The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties.
Fiche publication
Date publication
juillet 1989
Journal
The EMBO journal
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHAMBON Pierre, Dr METZGER Daniel, Dr TORA Laszlo
Tous les auteurs :
Tora L, Mullick A, Metzger D, Ponglikitmongkol M, Park I, Chambon P
Lien Pubmed
Résumé
We demonstrate here that the human oestrogen receptor (hER) cDNA clone pOR8 obtained from MCF-7 cells contains an artefactual point mutation which results in the substitution of a valine for a glycine at amino acid position 400 (Gly-400----Val-400). This mutation in the hormone binding domain of the cloned hER destabilizes its structure and decreases its apparent affinity for oestradiol at 25 degrees C, but not at 4 degrees C, when compared with the wild-type hER with a Gly-400.
Mots clés
Base Sequence, Breast Neoplasms, Cell Line, Cloning, Molecular, DNA, Neoplasm, genetics, DNA-Directed DNA Polymerase, Female, Gene Amplification, Genes, Glycine, Humans, Kinetics, Molecular Sequence Data, Mutation, Oligonucleotide Probes, Receptors, Estrogen, genetics, Valine
Référence
EMBO J.. 1989 Jul;8(7):1981-6