Promoter specificity of the two transcriptional activation functions of the human oestrogen receptor in yeast.
Fiche publication
Date publication
juin 1992
Journal
Nucleic acids research
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHAMBON Pierre, Dr METZGER Daniel
Tous les auteurs :
Metzger D, Losson R, Bornert JM, Lemoine Y, Chambon P
Lien Pubmed
Résumé
We have previously demonstrated that the human oestrogen receptor (hER) contains two transcriptional activation functions located in the N-terminal region (TAF-1) and in the hormone binding domain (TAF-2), which can act both independently and synergistically in a promoter- and cell-specific manner in animal cells. We have also demonstrated that hER can activate transcription from chimaeric oestrogen-responsive GAL1 promoters in yeast, and shown that transcriptional activation was due to TAF-1, whereas TAF-2 showed little, if any, transcriptional activity on these promoters. By using a more complex promoter derived from the URA3 gene, we now show that TAF-2 is also active in yeast, and that the activities of TAF-1 and TAF-2 are promoter-context-specific in yeast. We also confirm that the agonistic activity of 4-hydroxytamoxifen (OHT) can be ascribed to the activity of TAF-1.
Mots clés
Base Sequence, Estrogens, pharmacology, Gene Expression Regulation, Fungal, drug effects, Molecular Sequence Data, Oligodeoxyribonucleotides, chemistry, Promoter Regions, Genetic, Receptors, Estrogen, genetics, Regulatory Sequences, Nucleic Acid, Saccharomyces cerevisiae, genetics, Tamoxifen, analogs & derivatives, Transcription Factors, genetics, Transcription, Genetic, drug effects
Référence
Nucleic Acids Res.. 1992 Jun;20(11):2813-7