A chimeric Cre recombinase inducible by synthetic,but not by natural ligands of the glucocorticoid receptor.

Fiche publication


Date publication

septembre 1998

Journal

Nucleic acids research

Auteurs

Membres identifiés du Cancéropôle Est :
Pr CHAMBON Pierre, Dr METZGER Daniel


Tous les auteurs :
Brocard J, Feil R, Chambon P, Metzger D

Résumé

We have developed a new ligand-dependent chimeric recombinase (Cre-GRdex) by fusing the site-specific Cre recombinase to the ligand binding domain (LBD) of a mutant human glucocorticoid receptor (GRdex). The synthetic glucocorticoid receptor (GR) ligands dexamethasone, triamcinolone acetonide and RU38486efficiently induce recombinase activity in F9 murine embryonal carcinoma cells expressing constitutively Cre-GRdex. In contrast, no recombinase activity was detected in the absence of ligand or in the presence of the natural GR ligands corticosterone, cortisol or aldosterone. Moreover, physiological concentrations of these natural GR ligands do not affect Cre-GRdexrecombinase activity induced by dexamethasone. Thus, as previously shown using Cre-oestrogen receptor (ER) fusion proteins, Cre-GRdexmight be useful for achieving loxP site-directed mutagenesis in cultured cells and spatio-temporally controlled somatic cell mutagenesis in transgenic mice.

Mots clés

Aldosterone, pharmacology, Animals, Corticosterone, pharmacology, Dexamethasone, pharmacology, Glucocorticoids, pharmacology, Humans, Hydrocortisone, pharmacology, Integrases, biosynthesis, Ligands, Mice, Mifepristone, pharmacology, Mutation, Receptors, Glucocorticoid, biosynthesis, Recombinant Fusion Proteins, biosynthesis, Recombination, Genetic, Transcriptional Activation, Triamcinolone Acetonide, pharmacology, Viral Proteins

Référence

Nucleic Acids Res.. 1998 Sep;26(17):4086-90