Impaired adipogenesis and lipolysis in the mouse upon selective ablation of the retinoid X receptor alpha mediated by a tamoxifen-inducible chimeric Cre recombinase (Cre-ERT2) in adipocytes.
Fiche publication
Date publication
janvier 2001
Journal
Proceedings of the National Academy of Sciences of the United States of America
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHAMBON Pierre, Dr METZGER Daniel
Tous les auteurs :
Imai T, Jiang M, Chambon P, Metzger D
Lien Pubmed
Résumé
Retinoid X receptor alpha (RXRalpha) is involved in multiple signaling pathways, as a heterodimeric partner of several nuclear receptors. To investigate its function in energy homeostasis, we have selectively ablated the RXRalpha gene in adipocytes of 4-week-old transgenic mice by using the tamoxifen-inducible Cre-ERT2 recombination system. Mice lacking RXRalpha in adipocytes were resistant to dietary and chemically induced obesity and impaired in fasting-induced lipolysis. Our results also indicate that RXRalpha is involved in adipocyte differentiation. Thus, our data demonstrate the feasibility of adipocyte-selective temporally controlled gene engineering and reveal a central role of RXRalpha in adipogenesis, probably as a heterodimeric partner for peroxisome proliferator-activated receptor gamma.
Mots clés
Adipocytes, drug effects, Adipose Tissue, drug effects, Animals, Body Temperature, drug effects, Body Weight, drug effects, Cell Differentiation, drug effects, Dimerization, Enzyme Induction, drug effects, Fasting, Histocytochemistry, Hypothermia, chemically induced, Integrases, genetics, Lipolysis, drug effects, Mice, Mice, Transgenic, Mutagenesis, drug effects, Obesity, chemically induced, RNA, Messenger, genetics, Receptors, Cytoplasmic and Nuclear, genetics, Receptors, Estrogen, genetics, Receptors, Retinoic Acid, deficiency, Recombinant Fusion Proteins, Retinoid X Receptors, Sodium Glutamate, pharmacology, Stem Cells, drug effects, Tamoxifen, pharmacology, Time Factors, Transcription Factors, deficiency, Viral Proteins
Référence
Proc. Natl. Acad. Sci. U.S.A.. 2001 Jan;98(1):224-8