Temporally controlled site-specific mutagenesis in the germ cell lineage of the mouse testis.
Fiche publication
Date publication
février 2003
Journal
Biology of reproduction
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHAMBON Pierre, Dr METZGER Daniel
Tous les auteurs :
Weber P, Schuler M, Gérard C, Mark M, Metzger D, Chambon P
Lien Pubmed
Résumé
We have obtained a PrP-Cre-ER(T) transgenic mouse line (28.8) that selectively expresses in testis the tamoxifen-inducible Cre-ER(T) recombinase under the control of a mouse Prion protein (PrP) promoter-containing genomic fragment. Cre-ER(T) is expressed in spermatogonia and spermatocytes, but not in Sertoli and Leydig cells. We also established reporter PrP-L-EGFP-L transgenic mice harboring a LoxP-flanked enhanced green fluorescent protein (EGFP) Cre reporter cassette under the control of the same PrP promoter-containing genomic fragment that exhibits prominent EGFP expression in brain and testis. Using the PrP-L-EGFP-L as well as other Cre-reporter mice, we demonstrate that tamoxifen administration efficiently and selectively induces Cre-mediated recombination in the germ cell lineage. The established PrP-Cre-ER(T) line should provide a valuable tool for studying functions of germ cell-expressed genes involved in spermatogenesis.
Mots clés
Animals, Brain, metabolism, Cell Lineage, Estrogen Antagonists, pharmacology, Genes, Reporter, Green Fluorescent Proteins, Integrases, genetics, Luminescent Proteins, genetics, Male, Mice, Mice, Transgenic, Mutagenesis, Site-Directed, Mutation, Pregnancy Proteins, genetics, Protein Structure, Tertiary, genetics, Receptors, Estrogen, genetics, Recombinant Fusion Proteins, genetics, Recombination, Genetic, drug effects, Spermatozoa, physiology, Tamoxifen, pharmacology, Testis, cytology, Time Factors, Tissue Distribution, Viral Proteins, genetics
Référence
Biol. Reprod.. 2003 Feb;68(2):553-9