Bottom-Up Two-Dimensional Electron-Capture Dissociation Mass Spectrometry of Calmodulin.
Fiche publication
Date publication
octobre 2017
Journal
Journal of the American Society for Mass Spectrometry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DELSUC Marc-André
Tous les auteurs :
Floris F, van Agthoven MA, Chiron L, Wootton CA, Lam PYY, Barrow MP, Delsuc MA, O'Connor PB
Lien Pubmed
Résumé
Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry technique that allows data-independent fragmentation of all precursors in a mixture without previous isolation, through modulation of the ion cyclotron frequency in the ICR-cell prior to fragmentation. Its power as an analytical technique has been proven particularly for proteomics. Recently, a comparison study between 1D and 2D MS has been performed using infrared multiphoton dissociation (IRMPD) on calmodulin (CaM), highlighting the capabilities of the technique in both top-down (TDP) and bottom-up proteomics (BUP). The goal of this work is to expand this study on CaM using electron-capture dissociation (ECD) 2D MS as a single complementary BUP experiment in order to enhance the cleavage coverage of the protein under analysis. By adding the results of the BUP 2D ECD MS to the 2D IRMPD MS analysis of CaM, the total cleavage coverage increased from ~40% to ~68%. Graphical abstract ᅟ.
Mots clés
Bottom up proteomics, Electron capture dissociation, Tandem mass spectrometry, Two-dimensional mass spectrometry
Référence
J. Am. Soc. Mass Spectrom.. 2017 Oct;: